Identification of main and epistatic QTLs for grain yield related traits in a recombinant inbred lines population of rice (Oryza sativa L.)
نویسندگان
چکیده مقاله:
Rice yield as a complex trait is the main target in most rice breeding programs. To map the main and epistatic QTLs controlling grain yield and yield components, an experiment was carried out using a 129 F6 recombinant inbred lines population (IRA population) originated from a cross between Alikazemi / IR67017-180-2-1-2, in 2015 growing season in two locations, Rasht and Tonekabon, Iran. The experimental design was augmented design arrangment with five check cultivars in randomized complete block design with five replications. Analysis of variance showed that the linkage map consisted of 87 Single Sequence Repeats (SSRs) covering 1356.0 cM of rice genome in 12 linkage groups with an average distance of 15.58 cM spamming two markers. The results of combined analysis of variance for two locations, using composite interval mapping method, identified a total of 13 main QTLs on rice chromosomes for five measured traits. The qTN3 with 13.9% for tiller number per plant, the qFG4 with 11.6% for filled grain per panicle and the qGY6 with 15.6% for grain yield had significant positive additive effect. Furthermore, the qSG1 with 19.9% phenotypivc variation had a negative additive effect on the number of unfilled grain.panicle-1. These finding suggest that these QTLs can be used in rice breeding programs for improving grain yield. The interaction between additive effect (A) of QTLs and environment (E) was significant on grain yield and number of unfilled grain.panicle-1, but it was not significan on other traits. A total of 18 QTL pairs with significant additive × additive (AA) epistatic effect were identified for all traits. The highest epistasic effects were related to grain yield and number of unfilled grain.panicle-1 with six pairs of QTLs for each of these traits. Only one of the epistatic effects between qSG1-2 and qSG6 had significant AAE effect with a R2aae = of 3.4%. In addition, some QTLs were identified as three gene clusters controlling the grain yield, number of tiller.plant-1 and number of filled grain.panicle-1. Furthermore, five microsatellite markers including RM7551, RM8218-RM3417 and RM5302- RM283 0.2 to 5 cM distance from were identified as linked markers with qGY6, qFG4 and qSG1, respectively. These markers can be considered in the marker-assisted rice breeding program.
منابع مشابه
Evaluation of drought tolerance in rice (Oryza sativa L.) cultivars and recombinant inbred lines
In order to study the adaptation of rice lines to drought stress and to identify the tolerant and sensitive lines, 150 pure lines in RIL population each derived from a cross between two Iranian rice varieties Gharib and Sepidroud were investigated. Augment design based on the randomized complete block design (RCBD) was used with 6 check cultivars and 4 replications in 2013-2014 growing season. ...
متن کاملAnalysis of QTLs for panicle exsertion and its relationship with yield and yield-related traits in rice (Oryza sativa L.).
Panicle exsertion (PE) is an important morphological trait that is closely associated with spikelet fertility and grain yield. To understand the genetic basis of PE and its relationships with yield and yield-related traits, a recombinant inbred population consisting of 240 lines derived from a cross between an Indica cultivar 'Kasalath' and a Japonica germplasm 'TD70', was studied over two year...
متن کاملResponse of Iranian Rice Recombinant Inbred Lines (Oryza sativa L.) to Salt Stress in Seedling Stage
Study of the morphological genetic diversity of 114 lines, which have been derived from the crosses between Tarom Mahalli and Khazar cultivars, has been performed at seedling stage as completely randomized design at the normal condition and salinity stress of 8 dS.m-1 in a hydroponic system. Significant differences were detected between genotypes for all traits. Mean of comparison demonstrated ...
متن کاملGenetic diversity analysis of recombinant inbred lines of rice (Oryza sativa L.) using microsatellite markers
Estimation of genetic diversity is an important factor in germplasm conservation and characterization. In rice breeding programs, genetic diversity information on specific regions of genome can be very useful for the application of marker assisted selection (MAS) and for gene mapping. A total of 152 rice lines were considered for breeding programs using microsatellites (SSR) technique. The tota...
متن کاملMapping QTLs for Agronomic Traits in Rice Under Water Stress Condition Using Iranian Recombinant Inbred Lines Population
In the current study, a set of 96 recombinant inbred lines (RIL) at F8, derived from a cross between two varieties, Anbarbu (sensitive to drought stress) and Sepidroud (tolerant to drought stress) were used. The experiment was performed at Gonbad Kavous located in the Golestan province of Iran in 2010–2011 using two augmented designs at normal and stress conditions, separately. The RIL populati...
متن کاملidentification of gene loci (qtls) controlling grain yield and yield components in rice (oryza sativa l.)
to identify qtls controlling grain yield and yield components, an f2 population consisting of 188 plants derived from the cross between two rice pure lines, binam and kadous, were grown in research field of faculty of agricultural sciences, university of guilan, rasht, iran, in 2008, and were recorded for 9 important traits related to grain yield. to construct linkage map, 85 marker of ssrs and...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 21 شماره 4
صفحات 368- 385
تاریخ انتشار 2020-02
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
کلمات کلیدی برای این مقاله ارائه نشده است
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023